Foundation for Biomedical Research and Innovation
細胞療法研究開発センター
研究

細胞加工、細胞培養、細胞規格に関する研究

弊センターでは、細胞治療で使われる細胞の研究開発をめざし、多能性幹細胞の安全性に関する前臨床試験を国からの委託事業として受託し、また多能性幹細胞や体性幹細胞の細胞規格、細胞培養法、細胞培養施設の設計等に関する研究を公的研究機関や企業とも実施しています。

主要論文リスト

2012-2014

.R Stahl, G Vanmeerbeeck, G Lafruit, R Huys, V Reumers, A Lambrechts, C K Liao, C C Hsiao, M Yashiro, M Takemoto, T Nagata, S Gomi, K Hatabayashi, Y Oshima, S Ozaki, N Nishishita, S Kawamata., “Lens-free digital in-line holographic imaging for wide field-of-view, high resolution and real-time monitoring of complex microscopic objects", SPIE 2014, 1-10.

A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. Imaizumi K, Nishishita N, Muramatsu M, Yamamoto T, Takenaka C, Kawamata S*, Kobayashi K, Nishikawa SI, Akuta T* PLOS ONE [PONE-D-13-33269R1] [EMID:6a0dda29a950b6c2] 2014 Feb.11.

Tumorigenicity studies of induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium (RPE) for the treatment of age-related macular degeneration. Kanemura H, Go MJ, Shikamura M, Nishishita N, Sakai N, Kamao H, Mandai M, Morinaga C, Takahashi M, Kawamata S. PLOS ONE [PONE-D-13-33269R1] [EMID:b35ee3590307985b] 2014 Jan 14;9(1):e85336.

Local Transplantation of Granulocyte Colony Stimulating Factor-Mobilized CD34+ Cells for Patients With Femoral and Tibial Nonunion: Pilot Clinical Trial. Kuroda R, Matsumoto T, Niikura T, Kawakami Y, Fukui T, Lee SY, Mifune Y, Kawamata S, Fukushima M, Asahara T, Kawamoto A, Kurosaka M. Stem Cells Transl Med. 2014 Jan;3(1):128-34. doi: 10.5966/sctm.2013-0106. Epub 2013 Dec 4.

Control of angiogenesis by VEGF and endostatin-encapsulated protein microcrystals and inhibition of tumor angiogenesis. Goichi Matsumoto, Rie Hirohata, Kousuke Hayashi, Yoko Sugimoto, Eiji Kotani,Junji Shimabukuro, Tomoko Hirano, Yumiko Nakajima, Shin Kawamata, Hajime Mori 2013 Biomaterials. 2014 Jan;35(4):1326-33.

Pigment Epithelium-Derived Factor Secreted from Retinal Pigment Epithelium Facilitates Apoptotic Cell Death of iPSC. Hoshimi Kanemura, Masahiro J. Go, Naoki Nishishita, Noriko Sakai, Hiroyuki Kamao, Yoji Sato, Masayo Takahashi, Shin Kawamata, Scientific Reports 2013 3: 2334

Direct binding of Grb2 plays important roles in development of myeloproliferative 3 disease induced by ETV6/FLT3 K Chonabayashi, M Hishizawa, S Kawamata, T Hori, T Uchiyama, Leukemia 2013 Jun;27(6):1433-6.

Bone regeneration by polyhedral microcrystals from silkworm virus. Goichi Matsumoto, Yukihiko Kinoshita, Duverney Arias, Eiji Kotani, Shin Kawamata Scientific Reports 2012;2:935

Generation of virus-free induced pluripotent stem cell clones on a synthetic matrix via a single cell subcloning in the naïve state Naoki Nishishita, Masayuki Shikamurab, Chiemi Takenaka, Nozomi Takadab, Noemi Fusaki, and Shin Kawamata, PLoS ONE, 2012;7(9). PMID 23129529

Highly Sensitive In Vitro Methods for Detection of Residual Undifferentiated Cells in Retinal Pigment Epithelial Cells Derived from Human iPS Cells. Kuroda T, Yasuda S, Kusakawa S, Hirata N, Kanda Y, Suzuki K, Takahashi M, Nishikawa S, Kawamata S, Sato Y. PLoS ONE. 2012;7(5):e37342. PMID: 22615985

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T. Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti‐inflammatory macrophage and T lymphocyte to cells with regenerative potential. J Am Heart Assoc. 2014;3:e000743. Originally published June 25, 2014.

○Fukui T, Mifune Y, Matsumoto T, Shoji T, Kawakami Y, Kawamoto A, Ii M, Akimaru H, Kuroda T, Horii M, Yokoyama A, Alev C, Kuroda R, Kurosaka M, Asahara T. Superior potential of CD34-positive cells compared to total mononuclear cells for healing of nonunion following bone fracture. Cell Transplant. 2014 May 2. [Epub ahead of print]

○Nakamura T, Torimura T, Iwamoto H, Kurogi J, Inoue H, Hori Y, Sumie S, Fukushima N, Sakata1 M, Koga H, Abe M, Ikezono Y, Hashimoto O, Ueno T, Oho K, Okamura T, Okuda S, Kawamoto A, Ii M, Asahara T, Sata M. CD34+ cell therapy is safe and effective in slowing the decline of hepatic reserve function in patients with decompensated liver cirrhosis. J Gastroenterol Hepatol. 2014 Apr 14. [Epub ahead of print]

○Kuroda R, Matsumoto T, Niikura T, Kawakami Y, Fukui T, Lee SY, Mifune Y, Kawamata S, Fukushima M, Asahara T, Kawamoto A, Kurosaka M. Local transplantation of granulocyte colony stimulating factor-mobilized CD34+ cells for patients with femoral and tibial nonunion: Pilot clinical trial. Stem Cells Transl Med. 2014;3(1):128-34.

○Fujita Y, Kinoshita M, Furukawa Y, Nagano T, Hashimoto H, Hirami Y, Kurimoto Y, Arakawa K, Yamazaki K, Okada Y, Katakami N, Uno E, Matsubara Y, Fukushima M, Nada A, Losordo DW, Asahara T, Okita Y, Kawamoto A. Phase II clinical trial of CD34+ cell therapy to explore endpoint selection and timing in patients with critical limb ischemia. Circ J. 2014;78(2):490-501.

Matsuda T, Miyagawa S, Fukushima S, Kitagawa-Sakakida S, Akimaru H, Horii-Komatsu M, Kawamoto A, Saito A, Asahara T, Sawa Y. Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model. Circ J. 2014;78(1):222-231.

Kawakami Y, Ii M, Matsumoto T, Kawamoto A, Kuroda R, Akimaru H, Mifune Y, Shoji T, Fukui T, Asahi M, Kurosaka M, Asahara T. A small interfering RNA targeting Lnk accelerates bone fracture healing with early neovascularization. Lab Invest. 2013;93(9):1036-53.

Kamata S, Miyagawa S, Fukushima S, Nakatani S, Kawamoto A, Saito A, Harada A, Shimizu T, Daimon T, Okano T, Asahara T, Sawa Y. Improvement of cardiac stem cell-sheet therapy for chronic ischemic injury by adding endothelial progenitor cell transplantation: analysis of layer-specific regional cardiac function. Cell Transplant. 2013 Apr 3. [Epub ahead of print]

Kamei N, Kwon SM, Alev C, Nakanishi K, Yamada K, Masuda H, Ishikawa M, Kawamoto A, Ochi M, Asahara T. Ex-vivo expanded human blood-derived CD133+-cells promote repair of injured spinal cord. J Neurol Sci. 2013; 328(1-2):41-50.

Mifune Y, Matsumoto T, Murasawa S, Kawamoto A, Kuroda R, Shoji T, Kuroda T, Fukui T, Kawakami Y, Kurosaka M, Asahara T. Therapeutic superiority for cartilage repair by CD271 positive marrow stromal cell transplantation. Cell Transplant. 2013; 22(7):1201-11.

Kamei N, Kwon SM, Kawamoto A, Ii M, Ishikawa M, Ochi M, Asahara T. Contribution of bone marrow-derived endothelial progenitor cells to neovascularization and astrogliosis following spinal cord injury. J Neurosci Res. 2012;90(12):2281-92.

Kawakami Y, Ii M, Alev C, Kawamoto A, Matsumoto T, Kuroda R, Shoji T, Fukui T, Masuda H, Akimaru H, Mifune Y, Kuroda T, Horii M, Yokoyama A, Kurosaka M, Asahara T. Local transplantation of ex vivo expanded bone marrow-derived CD34 positive cells accelerates fracture healing. Cell Transplant. 2012;21(12):2689-709.

○Kinoshita M, Fujita Y, Katayama M, Baba R, Shibakawa M, Yoshikawa K, Katakami N, Furukawa Y, Tsukie T, Nagano T, Kurimoto Y, Yamasaki K, Handa N, Okada Y, Kuronaka K, Nagata Y, Matsubara Y, Fukushima M, Asahara T, Kawamoto A. Long-term clinical outcome after intramuscular transplantation of granulocyte colony stimulating factor-mobilized CD34 positive cells in patients with critical limb ischemia. Atherosclerosis. 2012; 224(2): 440-445.

Masuda H, Iwasaki H, Kawamoto A, Akimaru H, Ishikawa M, Ii M, Shizuno T, Sato A, Ito R, Horii M, Ishida H, Kato S, Asahara. T. Development of serum-free quality and quantity control culture of colony-forming endothelial progenitor cell for vasculogenesis. Stem Cells Trans Med. 2012;1(2):160-171.

Kamei N, Kwon SM, Ishikawa M, Ii M, Nakanishi K, Yamada K, Hozumi K, Kawamoto A, Ochi M, Asahara T. Endothelial progenitor cells promote astrogliosis following spinal cord injury through Jagged1-dependent Notch signaling. J Neurotrauma. 2012; 29(9):1758-69.

Fukui T, Ii M, Shoji T, Matsumoto T, Mifune Y, Kawakami Y, Akimaru H, Kawamoto A, Kuroda T, Saito T, Tabata Y, Kuroda R, Kurosaka M, Asahara T. Therapeutic effect of local administration of low dose simvastatin-conjugated gelatin hydrogel for fracture healing. J Bone Miner Res. 2012; 27(5):1118-31.

Kato A, Imai Y, Aoki K, Tabata S, Matsushita A, Hashimoto H, Takahashi T, Ishikawa T.  Serum IgA level, monocyte count, and international prognostic index are independently associated with overall survival in patients with HTLV-I-negative nodal peripheral T cell lymphoma. Ann Hematol. 2014 Feb 14.

Arima H, Ono Y, Tabata S, Matsushita A, Hashimoto H, Ishikawa T, Takahashi T. Successful allogeneic hematopoietic stem cell transplantation with reduced-intensity conditioning for B-cell prolymphocytic leukemia in partial remission. Int J Hematol. 2014 Jan 28.

Fujita Y, Kinoshita M, Furukawa Y, Nagano T, Hashimoto H, Hirami Y, Kurimoto  Y, Arakawa K, Yamazaki K, Okada Y, Katakami N, Uno E, Matsubara Y, Fukushima M, Nada A, Losordo DW, Asahara T, Okita Y, Kawamoto A. Phase II Clinical Trial of CD34+ Cell Therapy to Explore Endpoint Selection and Timing in Patients With Critical Limb Ischemia. Circ J. 2014 Jan 24;78(2):490-501. Epub 2013 Nov 21.

Aoki K, Arima H, Kato A, Hashimoto H, Tabata S, Matsushita A, Ishikawa T. Human herpes virus 6-associated myelitis following allogeneic bone marrow transplantation. Ann Hematol.